The picture was taken one year prior to Starr joining 'The Beatles'
One of the first photographs of The Beatles playing in Liverpool's Cavern Club is set to be auctioned off, along with other recently discovered picture of the band during the beginning of their career.
Stephen Bailey - the manager of The Beatles Shop, in Liverpool - said the memorabilia sale includes a series of negatives brought into the Mathew Street store by a man who mentioned, "My father was a professional photographer and these might be of interest to you."
The man reportedly turned in a series of negatives with photos that ended up being in perfect condition. According to Bailey's findings, the image that features Best instead of Starr was taken on June 14, 1961, when the band played its first show upon returning from Hamburg.
Another shot which stands out features George Harrison, John Lennon, McCartney and Best posing with six girls.
Mr Bailey said he did some detective work on this photograph and found out it was taken on June 14, 1961, at St John's Hall, Tuebrook, Liverpool - the first date the band played after returning from Hamburg.
The Annual Liverpool Beatles Auction will be held in the Paul McCartney Auditorium at the Liverpool Institute of Performing Arts on Saturday, August 23.
"I am very excited about the auction. It just gets bigger and better every year," said Bailey.
Bailey said there would be 330 lots at the auction, including the photo without Starr, along with the first, second and third editions of Merseybeat magazine. He expects the pictures to bring in somewhere around 1,500 pounds and the magazines to go for 2,500 to 3,000 pounds.