Genome Editing Method Discovered That Does Not Cleave DNA

Posted: Aug 27 2016, 1:41pm CDT | by , in Latest Science News


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Genome Editing Method Discovered That Does Not Cleave DNA
Deaminase is attached by a linker to nuclease-deficient CRISPR/Cas9. Guide RNA recognizes the DNA sequence of target genome and the deaminase modifies the base of the unwound DNA. Credit: Kobe University

A genome editing method was discovered that does not cleave DNA. The researchers have really done it this time and they deserve all the praise in the world for this monumental achievement.

Kobe University researchers have developed Target-AID which is a genome editing technique that does not cleave DNA. Via a sophisticated editing methodology, the gene therapy of the future may just be here although in a prototypical form.

This is a potent tool for producing organisms having a lot of utility. Also research efforts regarding disease and drug genesis will bear fruit in the times to come.

The study was published online in the journal Science.

The team of researchers came from many institutes. Genome editing is a technique that can rearrange the genes of various organisms without letting any loose pieces or bits hang out.

This sub-field of genetic engineering has gotten more and more advanced with the passage of time. It has in fact become a truly revolutionary science and holds positive implications for medicine and life studies.

Genome editing uses artificial nuclease to snip the DNA strands at the designated point and to transform the gene while it is undergoing repair work.

This method is very efficient for biological procedures where genetic aims are normally a headache. Higher organisms such as plants and animals are ideal for such operations.

There is however a major flaw in the works somewhere. The question of whether the mutation will occur or not remains a moot point. Also cytotoxicity remains a blind spot in this process.

Thus we have two huge gaping holes in this otherwise useful methodology. That is where the present day research efforts have proved fruitful to the extreme.

The modification of genes is possible via target point mutation on a very operational basis. This was made possible via a synthesis of complex materials. The removal of nuclease activity from the CRISPR system is the gist of the dismantling job.

Artificial nuclease and the adding of deaminase are the name of the game. A deaminizing or base-changing enzyme is expressed via yeast cells and mammalian components.

As compared to erstwhile nuclease models, the cytotoxicity of this process is much less severe in its effects. Thus it is a good thing that got even better thanks to mankind’s intelligence and creativity.

The DNA is transformed without the snipping and cutting. The stress and strain that the cells underwent in the past has gone with the wind. Gene therapy will be a cinch thanks to this novel discovery.

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<a href="/latest_stories/all/all/20" rel="author">Sumayah Aamir</a>
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